Intrada Amino Acid

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Life Sciences Separation Column

World's First LC-MS Untargeted Amino Acid Analysis Column

FEATURES

Enables direct LC-MS analysis of amino acids without derivatization

Capable of separating isomers like Leu, Ile, and others

Protein constituent amino acids can be measured in 5-10 minutes

Suitable for amino acids, related compounds, dipeptides, etc.

High-throughput analysis in less than 1 minute possible depending on amino acid composition

The conventional methods of amino acid analysis had the following difficulties.

Post-labeling Methods: Utilizes ion-exchange modes, requiring complete separation of amino acids, leading to complex mobile phases and difficulties in shortening analysis time.
Pre-labeling Methods: Allows for short-time analysis using reversed-phase modes, but requires time and equipment for amino acid pre-labeling reactions. Additionally, labeling efficiency is susceptible to sample matrix interference.

Advantages of this Product

Our product allows selective detection of amino acids with different masses using LC-MS. As a pioneering column specialist, we have developed a dedicated stationary phase surface structure, a world-first, to leverage universal LC-MS.

Intrada Amino Acid column, coupled with universal LC-MS, enables rapid analysis without the complexity of labeling. Moreover, with unprecedented column size variations, it achieves high-throughput analysis in as little as 1 minute depending on amino acid compositions.

Its innovative design not only separates Leucine/Isoleucine but also accommodates a wide range of applications for amino acid-related compounds such as β- and γ-amino acid isomers and dipeptides.

PUBLICATIONS

U.S. FDA Publication

Real-Time Quantification of Amino Acids in Antibody Drug Production Media

Real-time quantification and supplementation of bioreactor amino acids to prolong culture time and maintain antibody product quality
https://aiche.onlinelibrary.wiley.com/doi/full/10.1002/btpr.2894

Tokyo Customs Laboratory Report No. 60

Amino acids analysis without derivatization by using liquid chromatography quadrupole time-of-flight mass spectrometer (LC-QTOF-MS)

Amino acids analysis without derivatization by using liquid chromatography quadrupole time-of-flight mass spectrometer (LC-QTOF-MS)
https://www.customs.go.jp/ccl_search/info_search/foodstuffs/r_60_03_j.pdf

IMTAKT Publication

Development of a Novel Amino Acids Analysis Column for LC-MS without Derivatization

BIOINDUSTRY (August,2014)

Life Sciences Separation Column

FEATURES

Advantages of this Product

PUBLICATIONS

Single Quad. MS (LC-MS)

Triple Quad. MS (LC-MS/MS)

TOF/MS

Orbitrap MS

[Applications for Actual Samples]

[Various Amino Acids]

[Amino Acids and Related Metabolites (Precursors)]

[Amino Acid Isomers]

[Low Molecular Weight Peptides]

>>>See all Intrada Amino Acid Application Database <<<

Lysinibacillus piscis sp. nov. isolated from the gut of mottled spinefoot Siganus fuscescens

Changes in TCA cycle and TCA cycle-related metabolites in plasma upon citric acid administration in rats

Glucose metabolism and pyruvate carboxylase enhance glutathione synthesis and restrict oxidative stress in pancreatic islets

Effects of induction on the farrowing process and piglet blood parameters at the time of farrowing

Nasopharyngeal metabolomics and machine learning approach for the diagnosis of influenza

Peripheral Hybrid CB1R and iNOS Antagonist MRI-1867 Displays AntiFibrotic Efficacy in BleomycinInduced Skin Fibrosis

Brain ethanol metabolism by astrocytic ALDH2 drives the behavioural effects of ethanol intoxication

Changes in sow milk lipidome across lactation occur in fatty acyl residues of triacylglycerol and phosphatidylglycerol lipids, but not in plasma membrane phospholipids

Nutritional evaluation of taurine and methionine in commercially available cat foods

Combining ion chromatography with mass spectrometry and inductively coupled plasma-mass spectrometry: Annual review 2020

2-Oxo-Imidazole-Containing Dipeptides Play a Key Role in the Antioxidant Capacity of Imidazole-Containing Dipeptides

High-Precision Sulfur Metabolomics Innovated by a New Specific Probe for Trapping Reactive Sulfur Species

Changes in sow milk lipidome across lactation occur in fatty acyl residues of triacylglycerol and phosphatidylglycerol lipids, but not in plasma membrane phospholipids

Hydroxyurea improves nitric oxide bioavailability in humanized sickle cell mice

Arid5a Promotes Immune Evasion by Augmenting Tryptophan Metabolism and Chemokine Expression

Comparisons of Chemical Composition, Flavor and Bioactive Substances between Korean and Imported Velvet Antler Extracts

α-Hydroxyisocaproic Acid Decreases Protein Synthesis but Attenuates TNFα/IFNγ Co-Exposure-Induced Protein Degradation and Myotube Atrophy via Suppression of iNOS and IL-6 in Murine C2C12 Myotube

Dynamin-related protein 1 inhibition reduces hepatic PCSK9 secretion

Fermented milk retains beneficial effects on skeletal muscle protein anabolism after processing by centrifugation and supernatant removal

Beneficial effects of the consumption of sun-dried radishes (Raphanus sativus cv. YR-Hyuga-Risou) on dyslipidemia in apolipoprotein E-deficient mice

Relationships among the Hexosamine Biosynthetic Pathway, Sleep Disturbance, and Cognition in Obstructive Sleep Apnea

Effects of suppressing protein structural changes on the excellent gelling properties of dried egg white via dry-heat treatment

Relative Late Gestational Muscle and Adipose Thickness Reflect the Amount of Mobilization of These Tissues in Periparturient Dairy Cattle

Distribution and quantitative analysis of homoanserine and its 2-oxo derivative in mouse tissues.

Mammary Development in Gilts at One Week Postnatal Is Related to Plasma Lysine Concentration at 24 h after Birth, but Not Colostrum Dose

Compound NSC84167 selectively targets NRF2-activated pancreatic cancer by inhibiting asparagine synthesis pathway

Increased solubility and functional properties of precipitated Alfalfa protein concentrate subjected to pH shift processes

Influence of Excessively High Temperatures on the Fruit Growth and Physicochemical Properties of Shiranuhi Mandarin in Plastic-Film Greenhouse Cultivation

Yield and Nutritional Characterization of Thirteen Quinoa (Chenopodium quinoa Willd.) Varieties Grown in North-West Europe - Part I

Flavor and Metabolite Profiles of Meat, Meat Substitutes, and Traditional Plant-Based High-Protein Food Products Available in Australia

Ulva fenestrata protein - Comparison of three extraction methods with respect to protein yield and protein quality

Homogenization in water improves the recovery rate of imidazole dipeptides in meat

Preparation of High GABA-Enriched Yeast Extract by Non-Saccharomyces Yeasts Isolated from Korean Traditional Fermented Soybean Product

Changes in TCA cycle and TCA cycle-related metabolites in plasma upon citric acid administration in rats

Characterization of Chinese hamster ovary cell culture feed media precipitate

Low temperature plasma irradiation products of sodium lactate solution that induce cell death on U251SP glioblastoma cells were identified

Impact of soy lecithin, zinc oxide, and methylsulfonylmethane, as excipient ingredients, on the bioaccessibility and intestinal transport of branched-chain amino acids from animal and plant protein mixtures

DEVELOPMENT AND VALIDATION OF AN LC-MS/MS ASSAY TO MEASURE GLUTATHIONE, GLUTATHIONE DISULFIDE, CYSTEINE, AND CYSTINE IN HUMAN BRAIN AND HUMAN GLIOBLASTOMA

Influence of Dietary Metformin on the Growth Performance and Plasma Concentrations of Amino Acids and Advanced Glycation End Products in Two Types of Chickens

Interactions between Amino Acids and Zirconia Modified with Ethylenediaminetetra(methylenephosphonic acid): Mechanistic Insights into the Selective Binding of Antibodies

Quantification of bovine plasma amino acids via liquid chromatography-electrospray ionizationmass spectrometry: Comparison of underivatized and precolumn derivatized methods

Circulating amino acids as fingerprints of visceral adipose tissue independent of insulin resistance: a targeted metabolomic research in women

Nitrogen Balance and Bioavailability of Amino Acids in Spirulina Diet-Fed Wistar Rats

Uncovering the Role of Gut Microbiota in Amino Acid Metabolic Disturbances in Heart Failure Through Metagenomic Analysis

Amino Acid Signature of Oxidative Stress in Patients with Type 2 Diabetes: Targeted Exploratory Metabolomic Research

Bacteroides spp. promotes branched-chain amino acid catabolism in brown fat and inhibits obesity

Aloe attenuates acute colonic inflammation in mice and enhances tight junction proteins in intestinal epithelial cells

Analysis of Amino acid Contents in Food for Revision of The Korean Food Composition Table

Changes in TCA cycle and TCA cycle-related metabolites in plasma upon citric acid administration in rats

オホーツクホンヤドカリPagurus ochotensisを食品として利用するための栄養成分分析

Exposure to chronic light-dark phase shifts during the prepartum nonlactating period attenuates circadian rhythms, decreases blood glucose, and increases milk yield in the subsequent lactation

Mass spectrometry-based stable-isotope tracing uncovers metabolic alterations in pyruvate kinase-deficient Aedes aegypti mosquitoes

Control of protein function through oxidation and reduction of persulfidated states

Impact of crop load on nitrogen uptake and reserve mobilisation in Vitis vinifera

Resistance exercise with anti-inflammatory foods attenuates skeletal muscle atrophy induced by chronic inflammation

Disulfide bond formation is not crucial for the heat-induced interaction between β-lactoglobulin and milk fat globule membrane proteins

Glucose-dependent partitioning of arginine to the urea cycle protects β-cells from inflammation

A Variant of SLC1A5 Is a Mitochondrial Glutamine Transporter for Metabolic Reprogramming in Cancer Cells

Effect of half-dry processing on umami components of vegetables and mushrooms

Effects of dietary intake of heat-inactivated Lactobacillus gasseri CP2305 on stress-induced behavioral and molecular changes in a subchronic and mild social defeat stress mouse model

Effects of administration of ascorbic acid and low-dose hydrocortisone after infusion of sublethal doses of lipopolysaccharide to horses

Involvement of NMDA receptors in tremor expression in Aspa/Hcn1 double-knockout rats

Isolation, Characterization and Structure Elucidation of a Novel Lantibiotic From Paenibacillus sp.

Free advanced glycation end product distribution in blood components and the effect of genetic polymorphisms

Comparison of Storage Stability, Volatile Compounds and Sensory Properties between Coarsely-and Finely-Marbled 1+ Grade Hanwoo Beef Loins

Photobiocatalysis by a Lytic Polysaccharide Monooxygenase Using Intermittent Illumination