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HPLC columns come in various
separation modes. The retention and separation performance depend on
the compatibility between the structure of the target analytes
(e.g., polarity, ionic character) and the stationary phase.
Imtakt offers the following types
of HPLC columns and separation modes. Please use this guide to
select the appropriate column for your application.
Reversed-Phase Mode (Alkyl and Phenyl
Stationary Phases)
Cadenza CD-C18
: High-precision reversed-phase separation of isomers.
Cadenza CL-C18
: ODS with controlled silanol content.
Cadenza CX-C18
: Suitable for basic compounds using formic acid in the mobile
phase.
Cadenza CW-C18
: Effective for large molecules above 1,000 Da.
Unison UK-C18
: Highly versatile ODS, compatible with aqueous to organic solvents.
Unison UK-C8
: Broad applicability for compounds ranging from high to low
polarity.
Unison UK-C1
: Excellent for highly hydrophobic, linear molecules.
Unison UK-Phenyl
: Ideal for complexes and structural isomers.
Dacapo DX-C18
: Suitable for analysis under alkaline mobile phase conditions.
Intrada WP-RP
: Allows easier elution of proteins compared to C4 wide-pore
columns.
Presto FF-C18
: The world’s only non-porous ODS column.
Reversed-Phase +
Ion-Exchange Mode
Scherzo SS-C18, SM-C18,
SW-C18 : Applicable to most ionic compounds.
Nardis
ND-RX : Enhances separation on phenyl stationary phases via dual
ion exchange.
Normal-Phase + Ion-Exchange Mode
Unison UK-Silica
: Normal-phase separation using acidic mobile phases.
Unison UK-Amino
: Versatile normal-phase separation, e.g., for sugars.
Nardis ND-NX
: Normal-phase separation using dual ion-exchange.
Reversed-Phase Mode (Direct Serum
Injection, High-Molecular-Weight Protein Exclusion, Surfactant
Retention)
Cadenza HS-C18
: Highly recommended for analyzing stabilizers in monoclonal
antibodies (mAbs).
Amino Acid LC-MS Analysis Column
Intrada Amino Acid
: Supports globally spreading methods for underivatized amino acid
LC-MS analysis.
Organic Acid LC-MS Analysis
Column
Intrada Organic Acid
: First silica-based column enabling underivatized organic acid
LC-MS analysis.
Macromolecular SEC-MS
Intrada SEC
: Silica-based column compatible with SEC-MS using volatile eluents.
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