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hplc column yazawa shool system diagram

The figure above shows a typical HPLC system.

"HPLC", an acronym for "High Performance Liquid Chromatography", saw widespread adoption beginning around 1970 with the advent of high-pressure pumps.

"Chromatography" refers to "compound separation", a technique for separating the components contained in a mixture to understand their properties and quantities. The crux of compound separation is the "separation column". The stationary phase within the column interacts with compounds, and differences in the interaction power result in variations in elution time (i.e., separation).

An HPLC system is primarily composed of three parts:

Delivery Section:
The mobile phase (the liquid moving within the column) is drawn from the reservoir by the pump and discharged, providing stable delivery to the column at a set flow rate.

Separation Section:
When a few to several hundred μL of sample solution containing solutes (in the case of analysis) is mixed into the mobile phase and "injected" into the column, a phenomenon occurs where the solutes interact on a molecular level with the stationary phase within the column, resulting in retention and separation.

Detection Section:
When the solutes separated and eluted from the column pass through the detector, they exhibit qualitative and quantitative responses. These responses are converted electrically, recording digital signals such as time-response or spectra.

In recent years, HPLC, with a mass spectrometer (MS) as the detector in the form of LC-MS, has become an indispensable tool for chemical analysis. It is used for identifying and quantifying trace elements in various fields such as pharmacology, food, environmental studies, and criminal investigation.

WG13 / YAZAWA Itaru,