Hybrid Column of PFP and Biphenyl
When conventional columns struggle with retention and separation,
MAIN SPECIFICATIONS
| Particle size: | 3μm |
| Pore size: | - |
| Stationary phase: | Pentafluorophenyl (PFP) + Biphenyl groups (phenyl-based stationary phase) |
| Separation mode: | Reversed phase |
| Target molecular weight: | Below 10kDa |
| pH range of use: | 1.5 - 8 |
| Column pressure resistance: | 3600psi / 250bar / 25MPa (1-6mm ID) |
Unlike ODS columns, Nardis NF-PFP/Biphenyl can separate tocopherol isomers (β and γ). This separation is attributed to π-dipole interactions between the PFP functional group and the phenyl and hydroxyl groups of tocopherols.
Thanks to its hybrid stationary phase consisting of PFP and Biphenyl ligands, Nardis NF-PFP/Biphenyl provides a unique selectivity distinct from conventional phenyl phases.
CAUTION
This product does not reproduce the same results as conventional PFP or Biphenyl columns under identical analytical conditions. Optimization of analytical parameters is required.
Since the stationary phase contains a small amount of cations, an appropriate ionic strength in the mobile phase (e.g., 50 mM ammonium acetate) is necessary for analyzing anionic analytes.
Characteristics of Nardis NF-PFP/Biphenyl - Phenyl-Based Reversed-Phase Column
Nardis NF-PFP/Biphenyl is a phenyl-based reversed-phase hybrid column incorporating both PFP and Biphenyl groups.
While the basic separation mode is reversed phase, the phenyl and fluorine functionalities impart unique selectivity compared to traditional phenyl stationary phases.
Mobile phases consist of water with organic solvents (acetonitrile, methanol, etc.) and, when necessary, acids (formic acid, acetic acid) or salts (ammonium formate, ammonium acetate).
As phenyl-based reversed-phase columns from Imtakt, comparative studies are recommended among:
Traditional phenyl stationary phase (USP L11)
Phenyl phase with dual ion-exchange ligands
This product
Comparative Selectivity of Phenyl-Based Phases
When analytes contain π-electrons or electronegative atoms such as O or N, π–π, ionic, and polar interactions with phenyl phases influence retention. As a result, identical analytical conditions yield different retention behaviors.
For structural isomers or analogs that are difficult to separate with conventional columns, improved resolution can often be achieved using one of the three phenyl-based columns.